Título: Diagnostic Microbiology and Infectious DiseaseImportada do Authenticus Pesquisar Publicações da Revista

Vol. 79 Nº 2

Páginas: 125-134

ISSN: 0732-8893

Editora: Elsevier

ISI Web of Knowledge - 10 Citações

ISI Web of Science

Scopus - 17 Citações

Pubmed / Medline

CORDIS: Ciências da Saúde

FOS: Ciências da engenharia e tecnologias

ID Authenticus: P-009-HX9

DOI: 10.1016/j.diagmicrobio.2014.03.002

Abstract (EN): The increasing occurrence of bacterial resistance to antibiotics has now reached a critical level. Finding antibiotic coadjuvants capable to inhibit the bacterial resistance mechanisms would be a valuable mid-term solution, until new classes of antibiotics are discovered. Selected plant alkaloids were combined with 5 antibiotics against 10 Staphylococcus aureus strains, including strains expressing distinct efflux pumps and methicillin-resistant S. aureus strains. The efficacy of each combination was assessed using the microdilution checkerboard, time-kill, Etest, and disc diffusion methods. The cytotoxicity of the alkaloids was evaluated in a mouse fibroblast cell line. Potentiation was obtained in 6% of all 190 combinations, especially with the combination of: ciprofloxacin with reserpine (RES), pyrrolidine (PYR), and quinine (QUIN); tetracycline with RES; and erythromycin with PYR. The highest cytotoxicity values were found for QUIN (half maximal inhibitory concentration [IC50] = 25 +/- 2.2 mg/L) and theophylline (IC50 = 100 +/- 4.7 mg/L).

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10