Resumo (PT):
Abstract (EN):
The thermodynamics and kinetics of DNA hybridization, i.e. the process of self-assembly of
one, two or more complementary nucleic acid strands, has been studied for many years. The
appearance of the nearest-neighbor model led to several theoretical and experimental papers
on DNA thermodynamics that provide reasonably accurate thermodynamic information
on nucleic acid duplexes and allow estimation of the melting temperature. Because there
are no thermodynamic models specifically developed to predict the hybridization temperature
of a probe used in a fluorescence in situ hybridization (FISH) procedure, the melting
temperature is used as a reference, together with corrections for certain compounds that are
used during FISH. However, the quantitative relation between melting and experimental FISH
temperatures is poorly described. In this review, various models used to predict the melting
temperature for rRNA targets, for DNA oligonucleotides and for nucleic acid mimics (chemically
modified oligonucleotides), will be addressed in detail, together with a critical assessment of
how this information should be used in FISH.
Idioma:
Inglês
Tipo (Avaliação Docente):
Científica
Nº de páginas:
12