Título: Journal of Biomedical Materials Research - Part AImportada do Authenticus Pesquisar Publicações da Revista

Vol. 105 Nº 2

Páginas: 491-497

ISSN: 1549-3296

Editora: Wiley-Blackwell

ISI Web of Knowledge - 16 Citações

Scopus - 16 Citações

INSPEC

ID Authenticus: P-00M-EZJ

DOI: 10.1002/jbm.a.35925

Abstract (EN): Implant-associated infections are caused by surface-adhering microorganisms persisting as biofilms, resistant to host defense and antimicrobial agents. Given the limited efficacy of traditional antibiotics, novel strategies may rely on the prevention of such infections through the design of new biomaterials. In this work, two antimicrobial agents applied to nanohydroxyapatite materialsnamely, chlorhexidine digluconate (CHX) and zinc oxide (ZnO) nanoparticleswere compared concerning their ability to avoid single- or dual-species biofilms of Staphylococcus aureus and Escherichia coli. The resulting biofilms were quantified by the enumeration of colony-forming units and examined by confocal microscopy using both Live/Dead staining and bacterial-specific fluorescent in situ hybridization. The sessile population arrangement was also observed by scanning electron microscopy. Both biomaterials showed to be effective in impairing bacterial adhesion and proliferation for either single- or dual-species biofilms. Furthermore, a competitive interaction was observed for dual-species biofilms wherein E. coli exhibited higher proliferative capacity than S. aureus, an inverse behavior from the one observed in single-species biofilms. Therefore, either nanoHA-CHX or nanoHA-ZnO surfaces appear as promising alternatives to antibiotics for the prevention of devices-related infections avoiding the critical risk of antibiotic-resistant strains emergence. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 491-497, 2017.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7