Título: INTERNATIONAL BIODETERIORATION & BIODEGRADATIONImportada do Authenticus Pesquisar Publicações da Revista

Vol. 86

Páginas: 34-41

ISSN: 0964-8305

Editora: Elsevier

ISI Web of Knowledge - 19 Citações

ISI Web of Science

Scopus - 21 Citações

FOS: Ciências exactas e naturais > Ciências biológicas

ID Authenticus: P-008-G3Z

DOI: 10.1016/j.ibiod.2013.03.024

Abstract (EN): The purpose of the present study was to assess the antibacterial activity of phenyl isothiocyanate (PITC), a synthetic isothiocyanate, on biofilms of Escherichia coli and Staphylococcus aureus. The effects of PITC on bacterial free energy of adhesion and motility were also investigated. Biofilm formation in 96-well polystyrene microtiter plates was quantified by crystal violet staining and the metabolic activity of those biofilms was assessed with alamar blue. The viability and culturability of the biofilm bacteria after exposure to PITC were determined. The highest removal and metabolic activity reduction of biofilms with PITC was around 90% for both bacteria. Treatment with PITC enabled 4.5 and 4.0 log(10) reductions of the number of viable cells for E. coli and S. aureus, respectively; and no colony forming units (CFUs) were detected. PITC also affected the adhesion process and motility of bacteria, greatly preventing biofilm formation. In conclusion, PITC enabled both biofilm prevention and control, promoting high biofilm removal and inactivation activities, suggesting that this compound is a promising disinfectant.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8