Título: International Journal of Food MicrobiologyImportada do Authenticus Pesquisar Publicações da Revista

Vol. 277

Páginas: 74-82

ISSN: 0168-1605

Editora: Elsevier

ISI Web of Knowledge - 2 Citações

Scopus - 22 Citações

INSPEC

ID Authenticus: P-00N-W6W

DOI: 10.1016/j.ijfoodmicro.2018.04.017

Resumo (PT):

Abstract (EN): This work investigated the effects of diamond-like carbon (DLC) coatings on the architecture and biocide reactivity of dual-species biofilms mimicking food processing contaminants. Biofilms were grown using industrial isolates of Escherichia coli and Pantoea agglomerans on bare stainless steel (SST) and on two DLC surface coatings (a-C:H:Si:O designated by SICON (R) and a-C:H:Si designated by SICAN) in order to evaluate their antifouling activities. Quantification and spatial organization in single- and dual-species biofilms were examined by confocal laser scanning microscopy (CLSM) using a strain specific labelling procedure. Those assays revealed that the E. cob isolate exhibited a higher adhesion to the modified surfaces and a decreased susceptibility to disinfectant in presence of P. agglomerans than alone in axenic culture. While SICON (R) reduced the short-term growth of E. con in axenic conditions, both DLC surfaces increased the E. coli colonization in presence of P. agglomerans. However, both modified surfaces triggered a significantly higher log reduction of E. coli cells within mixed-species biofilms, thus the use of SICON (R) and SICAN surfaces may be a good approach to facilitate the disinfection process in critical areas of food processing plants. This study presents a new illustration of the importance of interspecies interactions in surface-associated community functions, and of the need to evaluate the effectiveness of hygienic strategies with relevant multi-species consortia.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9